ammp-8与种植体黏膜炎

No abstract available

Background/Objectives: The long-term success of dental implants can be influenced by the material properties of abutments and their interaction with peri-implant tissues. This study investigates the impact of three abutment materials—titanium (Ti), zirconium oxide (Zr), and polymethylmethacrylate (PMMA)—on the inflammatory response in peri-implant sulcular fluid (PISF), using active-matrix metalloproteinase-8 (aMMP-8) as a biomarker. Methods: In this prospective, randomized clinical trial, 30 patients were assigned to Ti, Zr, or PMMA abutment groups. PISF samples were collected at predefined intervals over 12 months and analyzed for aMMP-8 levels using enzyme-linked immunosorbent assays (ELISA). Clinical parameters (probing depth, bleeding on probing, and plaque index) and radiographic assessments of bone resorption were also evaluated. Results: Two weeks after implant uncovering, baseline aMMP-8 levels varied significantly among materials, with Zr demonstrating the highest levels. Over time (2, 3, 6 and 12 months after implant uncovering), aMMP-8 levels decreased across all groups, with no significant differences observed at 12 months. Radiographic assessments indicated no statistically significant differences in bone resorption, with clinical parameters remaining comparable across all groups. Conclusions: Initial inflammatory responses to abutment materials may vary; however, all tested materials—Ti, Zr, and PMMA—showed long-term biocompatibility and supported healthy peri-implant tissue integration. These findings indicate that selecting any of the tested abutment materials does not significantly affect long-term peri-implant health.

The objective of this study was to investigate the effectiveness of testing for active matrix metalloproteinase‐8 (aMMP‐8) by a quantitative point‐of‐care (PoC), chairside lateral flow immunotest and azurocidin, in the peri‐implant sulcular fluid (PISF), as biomarkers for the presence or absence of peri‐implant diseases.

Active matrix metalloproteinase-8 (aMMP-8) is a promising biomarker candidate for the modern periodontal and peri-implant disease diagnostics utilizing the chairside/point-of-care oral fluid technologies. These rapid biomarker analysis technologies utilize gingival crevicular fluid (GCF), peri-implant sulcular fluid (PISF), or mouth rinse as the oral fluid matrices that can be collected patient-friendly and non-invasively without causing bacteremia. aMMP-8, but not total or latent proMMP-8, has been shown to be a relevant biomarker to be implemented to the latest 2017 classification system of periodontitis and peri-implantitis. Thus, aMMP-8 point-of-care-testing (POCT)—but not total or latent proMMP-8—can be conveniently used as an adjunctive and preventive diagnostic tool to identify and screen the developing and ongoing periodontal and peri-implant breakdown and disease as well as predict its episodic progression. Similarly, aMMP-8 POCT provides an important tool to monitor the treatment effect of these diseases, but also other diseases such as head and neck cancer, where it can identify and predict the rapid tissue destructive oral side-effects during and after the radiotherapy. Additionally, recent studies support aMMP-8 POCT benefitting the identification of periodontitis and diabetes as the escalating risk diseases for COVID-19 infection. Overall, aMMP-8 POCT has launched a new clinical field in oral medicine and dentistry, i.e., oral clinical chemistry.

Background: Osseointegrated dental implants have become increasingly common as a treatment option for missing teeth. Peri-implant infections are caused by bacterial plaque that may initiate an inflammatory release of cytokines, enhance accumulation of neutrophils in implant lesion, and trigger the production of matrix metalloproteinase-8 (MMP-8). MMP-8 is essential in inflammatory and degenerative processes of periodontal tissues and produced by activated cells. The purpose of this study was to detect the role of MMP-8 as a biomarker of active and aggressive peri-implant mucositis. Material and method: Eighty subjects (40 with peri-implant mucositis and 40 with successful and healthy peri-implant mucosa) were enrolled in this study. The 42 male and 38 female subjects were attended at AL-Karama and AL-Ma'amoun Specialized Dental Centers in Baghdad, Iraq from November 24, 2021 to May 25, 2022. Follow-up examinations were performed on patients to monitor the progression of disease. Peri-implant sulcular fluid was examined and identified using enzyme-linked immunosorbent assay technique for MMP-8. Results: Results showed that MMP-8 levels continue to rise after 3 weeks and are significantly higher in the patient group (P=0.00000) than the group with successful implants. Conclusion: MMP-8 can be used to reflect, associate, and predict clinical disease activity and progression of peri-implant mucositis properly.

The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth rinse, gingival crevicular fluid (GCF), and peri-implantitis fluid (PISF)) is demanding. Several hosts and microbial factors may influence their expression, release, and levels. The type of saliva/oral fluids utilized for the diagnostics affects the analysis. High sensitivity and specificities together with sophisticated methods and techniques are essential for valuable outcome. We describe here recently developed practical, convenient, inexpensive, noninvasive, and quantitative mouth rinse and PISF/GCF/chair-side/point-of-care (PoC) lateral-flow aMMP-8 immunoassays (PerioSafe and ImplantSafe/ORALyser) to detect, predict, and monitor successfully the course, treatment, and prevention of periodontitis and peri-implantitis, respectively. The tests have been independently and successfully validated to differentiate periodontal and peri-implant health and disease in Finland, Germany, Netherland, Sweden, Turkey, Nigeria, Malawi, and USA. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires additional studies utilizing these noninvasive screening, diagnostic, and preventive aMMP-8 PoC/chair-side technologies.

ABSTRACT Background: The use of photodynamic therapy (PDT) has been evaluated as an adjunctive technique for bacterial decontamination of implants with peri-implantitis. Given the controversies over the efficacy of the application of PDT to treat peri-implant diseases, the present clinical study aimed to evaluate the posttherapeutic clinical parameters and cytokine levels in peri-implant crevicular fluid in patients with peri-implant mucosal inflammation, receiving mechanical debridement (MD) alone or in association with PDT. Materials and Methods: In this double-blinded randomized clinical trial, 52 patients with peri-implant mucosal inflammation were selected and they were randomly assigned to 2 treatment groups: a MD group and an MD + PDT group using an 805 nm laser and indocyanine green (ICG). Although the decrease in bleeding on probing was the primary outcome, pocket depth, PUS, pain on probing, clinical attachment level, gingival recession, tumor necrosis factor-α, interleukin (IL)-1β, IL-6 and matrix metalloproteinase-8 were also evaluated at baseline, 2-week, and 3-month postintervention. Repeated measure analysis of variance was used to analyze inter-group differences and a P ≤ 0.05 was considered for significant differences between tested parameters. Results: Statistically significant improvements (P < 0.001) were detected for all variables after comparison of baseline data with those collected at each time interval of the study. Nevertheless, the inter-group comparisons of these variables between the baseline, 2-week, and 3-month intervals did not reveal any significant decrease in sites treated with either MD alone or MD + PDT. Conclusion: The application of PDT using 805-nm laser and ICG as an adjunct therapy to MD did not provide any additional improvements in the clinical or biologic parameters of peri-implant mucosal inflammation.

BACKGROUND Clinical probing is commonly recommended to evaluate peri-implant conditions. In a situation of peri-implant mucositis or peri-implantitis, the peri-implant seal healing from the disruption of soft tissue caused by probing has not yet been studied. This study aimed to investigate soft tissue healing after standardized clinical probing around osseointegrated implants with peri-implant mucositis in a dog model. METHODS Three transmucosal implants in each hemi-mandible of 6 dogs randomly assigned to the peri-implant healthy group or peri-implant mucositis group were probed randomly in the mesial or distal site as probing groups (PH or PM), the cross-sectional opposite sites as unprobed control groups. Histomorphometric measurements of implant shoulder (IS)-alveolar bone contact to the implant surface (BCI), apical termination of the junctional epithelium (aJE)-BCI, mucosal margin (MM)-BCI, and MM-aJE were performed at 1 day, 1 week and 2 weeks after probing. Apoptosis, proliferation, proinflammatory cytokines, and matrix metalloproteinases (MMPs) of peri-implant soft tissue were estimated by immunofluorescent analysis. RESULTS In PM group, apical migration of junctional epithelium was revealed by significantly decreased aJE-BCI from 1 day to 2 weeks in comparison to unprobed sites (P<0.05), while no significant differences were found in PH group. Immunofluorescent analysis showed higher levels of interleukin 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), MMP-1, and MMP-8, together with exaggerated apoptosis and proliferation of peri-implant soft tissue in PM group. CONCLUSION Within the limitations, standardized clinical probing might lead to apical migration of the junctional epithelium in a situation of peri-implant mucositis. This article is protected by copyright. All rights reserved.

AIM This randomized placebo-controlled clinical trial evaluated the effects of multispecies probiotic containing L. rhamnosus HN001TM , L. paracasei Lpc-37®, and B. lactis HN019TM as an adjunct to mechanical debridement (MD) on changes in bleeding on probing (BOP) in edentulous patients with peri-implant mucositis (PiM). MATERIALS AND METHODS Patients were randomly assigned to Test (Probiotic) or Control (Placebo) groups. All sites with PiM received MD and topical gel application (probiotic or placebo) at baseline and 12 weeks. After initial MD, patients consumed probiotic or placebo capsules twice a day for 12 weeks. Clinical (modified sulcus bleeding index - mSBI; modified plaque index - mPI; probing depth - PD; and BOP) and immunological parameters were collected at baseline and after 12 and 24 weeks. Data were statistically analyzed (p<0.05). RESULTS Thirty-six patients with PiM were recruited. The Test group presented higher prevalence (p<0.05) of cases of restored peri-implant health at 24 weeks than did the Control group (72.2% and 33.3%, respectively). No significant difference was observed between Test (n=18) and Control (n=18) groups for mPI and PD. mSBI % - score 0 was higher in the Test group than in the Control group at 24 weeks (p<0.05). When compared with baseline, both groups presented reduced BOP at 12 and 24 weeks (p<0.05). BOP was lower in the Test group than in the Control group at 12 (mean difference = -14.54%; 95% CI = -28.87/0.22; p=0.0163) and 24 (mean difference = -12.56%; 95% CI = -26.51/1.37; p=0.0090) weeks. At 24 weeks, only the Test group presented lower levels of IL-1β, IL-6, IL-8, and TNF-a than those at baseline (p<0.05). CONCLUSION The multispecies probiotic (administered locally and systemically) containing L. rhamnosus HN001TM , L. paracasei Lpc-37®, and B. lactis HN019TM as an adjunct to repeated MD promotes additional clinical and immunological benefits in the treatment of PiM in edentulous patients.

Background The present study aimed to evaluate whether non-surgical treatment interferes with clinical parameters and local patterns of osteo-immunoinflammatory mediators (IL-17 and TNF-α) and matrix metalloproteinase-8 (MMP-8) that are found in peri-implant crevicular fluid (PICF) and biofilms during the progression of peri-implant mucositis. Material/Methods We selected 30 patients with peri-implant caused mucositis before (MP) and after treatment (TP) and 30 healthy people (HP) for the analysis of IL-17, TNF-α cytokine, and MMP-8 production in PICF and for analysis of colonization dynamics of periodontopathogenic bacteria in supra- and subgingival plaque samples. The levels of IL-17 and MMP-8 concentrations in samples were assayed by enzymatic immunosorbent assay (ELISA) and TNF-α levels were determined by enzyme amplified sensitivity immunoassay (EASIA) method in PICF. The micro-IDent test was used to detect 11 species of periodontopathogenic bacteria in subgingival biofilm. Results We found significantly (P<0.001) higher levels of IL-17, TNF-α, and MMP-8 in the PICF of the MP and TP groups in comparison to the HP group. A significant association was found in MP associated with Parvimonas micra, as TNF-α in PICF was significantly higher (P=0.034) than in patients without Parvimonas micra. TNF-α levels in the samples of PICF showed a moderate correlation with clinical parameters, including plaque index (PI) (P=0.007) and MMP-8 levels (P=0.001), in the MP group. Conclusions Assessment of levels of inflammatory cytokines in PICF can aid in the identification of peri-implant mucositis, which can assist in early diagnosis, prevention, and treatment.

This study aimed to evaluate the correlation between epidermal growth factor (EGF) and receptor (EGFR) levels in different clinical stages of dental implant rehabilitation and trace mucositis development’s biological profile. Thirty-six participants from the Specialization in Implant Dentistry, Universidade Federal Fluminense, Brazil, were included in the study and underwent sample collection: inside the alveolar socket, immediately before implant placement (Group 1, n = 10); at the peri-implant crevicular fluid (PICF) during reopening (Group 2, n = 10); PICF from healthy peri-implant in function (Group 3, n = 8); and PICF from mucositis sites (Group 4, n = 18). Quantitative polymerase chain reaction (PCR) evaluated EGF/EGFR gene expression using the SYBR Green Master Mix detection system. The results showed that EGF expression in the peri-implant crevicular fluid was statistically different. There was a higher EGF expression for group C (peri-implant health) (p = 0.04) than for the other groups. Regarding EGFR, there was no statistical difference among the groups (p = 0.56). It was concluded that low levels of EGF gene expression in the peri-implant crevicular fluid are related to the development of peri-implant mucositis and the absence of mucosae sealing. There was no correlation between EGFR gene expression with health or mucositis.

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This communication article addresses currently available rapid non-invasive methods to screen and detect periodontitis and dental peri-implantitis. In this regard, oral fluid biomarkers have been researched extensively but self-reported oral health (SROH)-questionnaires have also been developed. Both alternatives may offer a quick and easy way to screen and detect diseased patients. Active matrix metalloproteinase (aMMP-8) is one of the most validated biomarkers for screening and detecting periodontal breakdown related to periodontitis and peri-implantitis and monitoring their treatment effects revealing successful, less- and non-successful treatment results. Currently available aMMP-8 lateral-flow technologies allow this kind of analysis, as demonstrated here, to be conducted quantitatively online and real-time as point-of-care/chairside testing in dental and even medical care settings. In this study, an aMMP-8 peri-implant sulcular fluid point-of-care-test diagnosed peri-implantitis and healthy implants far more accurately than bleeding-on-probing or the other biomarkers, such as polymorphonuclear (PMN)/neutrophil elastase, myeloperoxidase and MMP-9. Although, SROH-questionnaires allow screening in similar settings but they lack the information about the current disease activity of periodontitis and peri-implantitis, which is of essential value in periodontal diagnostics and treatment monitoring. Thus, both methods can be considered as adjunct methods for periodontitis and peri-implant diagnostics, but the value of oral fluid biomarkers analysis does not seem to be substitutable.

Running head Peri-implant maintenance with CHX Abstract Background: To assess the effect of chlorhexidine (CHX) chip application in patients with peri-implant mucositis as compared to CHX gel application. Methods: In peri-implant sites with mucositis, CHX gel was applied in the control group (GC) and CHX chips in the test group (CC) at baseline and after three months. At baseline and after six months, peri-implant pocket depths (PPD), bleeding-on-probing (BOP) and activated matrix metalloproteinase-8 (aMMP8) were assessed. Longitudinal changes were tested for inter-group differences. Results: Thirty-two patients were treated. BOP was more reduced (p = 0.006) in CC than in GC, with means and standard deviations of 46 ± 28% and 17 ± 27%, respectively. PPD was more reduced (p = 0.002) in CC than in GC with 0.65 ± 0.40 mm and 0.18 ± 0.32 mm, respectively. Regarding BOP, the percentages of improved, unchanged and worsened sites accounted for 32%, 61% and 7% in GC and 46%, 53% and 1% in CC, respectively. For probing pocket depth, the according values were 26%, 66% and 8% (GC) versus 57%, 38% and 5% (CC). Conclusions: During supportive therapy, repeated CHX chip application might resolve marginal peri-implant inflammation in terms of bleeding better than CHX gel.

Abstract Objective: Aim of present study was to evaluate gingival tissue samples obtained from healthy and diseased sites of teeth and dental implants in terms of hypoxia and collagenase activity. Methods: Four study groups were created as Group-1; healthy individuals (H), Group-2; periodontitis patients with stage 3 grade B (P), Group-3; patients with peri-implant mucositis. Group-4; patients with peri-implantitis (P-IMP). Plaque index (PI), gingival index (GI) and probing pocket depth (PPD) were recorded. Gingival and peri-implant mucosal biopsies were obtained. Fibroblast and inflammatory cells were counted. Hypoxia-inducible factor (HIF)-1α, prolyl hydroxylase (PH), matrix metalloproteinase (MMP)-8, tissue inhibitor of MMPs (TIMP)-1, cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) levels were determined via immunohistochemistry. Results: Healthy controls had highest fibroblast cell counts and lowest inflammatory cell counts compared to other groups. Peri-implantitis and periodontitis samples had similar fibroblast and inflammatory cell counts, while peri-implant mucositis had higher fibroblast cells and lowered inflammatory cells compared to periodontitis and peri-implantitis samples. HIF-1α, COX-2 and iNOS levels were lowest in healthy controls and increased in other groups. Peri-implant mucositis samples had significantly lower expressions of HIF-1α, COX-2 and iNOS compared to peri-implantitis and periodontitis groups. PH expressions were lower in periodontitis and peri-implantitis groups compared to healthy controls and peri-implant mucositis groups. MMP-8 levels were lower in healthy group compared to other groups while levels were similar in periodontitis, peri-implant mucositis and peri-implantitis groups. TIMP levels were similar in groups. Conclusion: Periodontitis, peri-implantitis, and peri-implant mucositis samples exhibited higher inflammation and lower fibroblast cell counts and tend to have increased tissue collagenase activity, hypoxia and inflammation compared to healthy samples.

Aim: The study was to evaluate the active matrix metalloproteinase-8 (aMMP-8) concentration in gingival crevicular fluid (GCF) and in peri-implant sulcular fluid (PISF) in healthy and diseased conditions, before and after non-surgical treatment, and to compare it with the various clinical parameters used to estimate the gingival and peri-implant inflammation. Methods: Plaque index/modified PI (PI/mPI), gingival index/simplified GI (GI/sGI), probing depth (PD), bleeding on probing index/modified BOPI (BOPI/mBOPI), radiographic bone loss/radiographic marginal bone loss (rBL/rMBL), and GCF/PISF samples were evaluated, before and 3 months after non-surgical treatment, GCF/PISF samples were analyzed by a chair-side mouth-rinse test (ImplantSafe®) in combination with a digital reader (ORALyzer®). Results: In all groups, aMMP-8 median levels were statistically higher in the PISF than in GCF and they did not change after treatment. Moreover, it was statistically higher in Group 3 (periodontitis/peri-implantitis) compared to the other groups. A positive correlation of the GCF/PISF and aMMP-8 median concentration was seen with increasing PD and BOPI/mBOPI values. A higher covariation of aMMP-8 mean levels in GCF with PD was found when compared to PISF levels. aMMP-8 mean levels in PISF expressed a higher covariation with increasing grades of sGI, rMBL, and BOPI while aMMP-8 GCF concentration established a better covariation with PD and PI. Conclusions: PISF of sites with peri-implant mucositis and peri-implantitis showed higher levels of aMMP-8 compared to sites with gingivitis and periodontitis. Compared to clinical indices, aMMP-8 concentration in GCF/PISF can be a beneficial adjunctive diagnostic tool for early identification and screening of the risk of peri-implant diseases. After non-surgical therapy, PISF aMMP-8 concentration remained mostly unchanged, while the GCF concentration of aMMP-8 significantly decreased.

Background. This study evaluated the gingival crevicular fluid (GCF) and Peri- implant crevicular fluid (PICF) concentrations of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and active metalloproteinase-8 (a-MMP-8) in sites with healthy conditions vs. sites affected by periodontitis (PER) and peri-implantitis (PIM). Methods. Periodontally healthy (PH) sites with PER, sites with peri-implant health (PIH), and sites with PIM were investigated intra-individually, according to the inclusion criteria of each group. Probing pocket depth (PPD), plaque index, gingival index, and the presence or absence of bleeding on probing (BoP) were evaluated. In GCF and PICF samples, IL-1β, IL-6, and TNF-α were quantified by ELISA Duoset® kit in combination with Ultramark® micro-ELISA digital reader; a-MMP8 concentration was analyzed by a chairside test (Perio/ImplantSafe®) in combination with a digital reader (ORALyzer®). Results. The concentrations of IL-6 and IL-1β, TNF-α, and a-MMP-8 were significantly higher in the PIM and PER sites compared to healthy sites (P<0.05). Significantly higher concentrations of IL-1β and a-MMP-8 were found in PIM vs. PER sites (P<0.05), while the concentrations of IL-6 and TNF-α did not differ between the PIM and PER groups (P>0.05). Conclusion. aMMP-8, IL-6, IL-1β, and TNF-α presented higher GCF/PICF concentrations in diseased periodontal and peri-implant sites. However, only the concentrations of IL-1β and a-MMP-8 were significantly higher in PIM than in PER sites.

Abstract Objectives  This study investigated the potential of testing for active matrix metalloproteinase-8 (aMMP-8) by a quantitative point-of-care (PoC), chairside, lateral flow immunotest as a biomarker for the presence or absence of peri-implant diseases. Materials and Methods  Eighty healthy patients with implants were recruited. The samples were collected from peri-implant sulcular fluid and quantitatively analyzed for aMMP-8. Clinical indices, which included probing depth, clinical attachment loss, bleeding on probing, and plaque, were recorded and radiographic assessments were performed. Statistical Analysis  Comparisons of aMMP-8 levels and clinical parameters were analyzed by the Kruskal–Wallis test and the pairwise post hoc Dunn–Bonferroni test. A receiver operating curve analysis was used to analyze the diagnostic ability of aMMP-8 and the correlation between aMMP-8 and probing depth of the sampled site was sought by Spearman's rho and the coefficient of determination ( R 2 ). Results  Statistical analysis revealed statistically significant differences of aMMP-8 levels between the healthy and the mucositis and peri-implantitis groups, and between the mucositis and the peri-implantitis groups. Increasing probing depths of the sampled site and aMMP-8 levels were significantly correlated. Conclusions  These data suggest that the aMMP-8 PoC test can be a beneficial adjunctive tool for early identification and screening of the risk of peri-implant diseases and progression.

The aim of this study was to investigate the utility of the active matrix metalloproteinase (aMMP‐8)‐point‐of‐care (PoC) test as a quantitative real‐time chair‐side diagnostic tool for peri‐implant diagnosis, as well as assess the potentially developing and ongoing risk relative to the traditional clinical methods.

Abstract Objective  This study aimed to evaluate the diagnostic sensitivity and specificity of the active matrix metalloproteinase-8 (aMMP-8) quantitative chairside point-of-care (PoC) lateral flow immunotest for peri-implant diseases, and it sought to correlate aMMP-8 levels with clinical parameters to determine its effectiveness as a biomarker for peri-implantitis. Materials and Methods  A cross-sectional study was conducted at the Department of Periodontology and Implant Biology, Aristotle University of Thessaloniki, Greece. Participants included systemically healthy individuals with at least one implant loaded for more than 1 year, who had not received periodontal treatment or antibiotics in the preceding 6 months. Exclusion criteria included diabetes and immune-compromising conditions. Peri-implant sulcular fluid (PISF) samples were collected from the mesiobuccal or distobuccal site of the implant. The quantitative chairside PoC lateral flow immunotest for peri-implant diseases (ImplantSafe test) and ORALyzer digital reader were used to analyze PISF, with results expressed in ng/mL. Clinical parameters such as bleeding on probing (BOP), probing depth (PD), recession (REC), and clinical attachment level (CAL) were measured at six sites per implant using a 15-mm scale periodontal probe. Results  No significant differences were found in age, gender distribution, or smoking status between the healthy/mucositis and peri-implantitis groups. The peri-implantitis group showed significantly higher mean percentages of BOP (57.58 ± 31.73 vs. 18.79 ± 24.17), PD (4.59 ± 1.22 mm vs. 2.94 ± 0.78 mm), and CAL (5.21 ± 1.72 mm vs. 3.05 ± 0.81 mm). aMMP-8 levels were also significantly higher in the peri-implantitis group (53.39 ± 49.70 vs. 22.03 ± 32.87). The diagnostic test demonstrated a sensitivity of 81.25% and specificity of 74.07%, with an area under the curve of 79.6%, indicating overall good accuracy in distinguishing between positive and negative conditions. Conclusion  The aMMP-8 is a promising biomarker for peri-implantitis, showing elevated levels in affected patients. The aMMP-8 chairside test demonstrates high diagnostic sensitivity and specificity, supporting its use in early detection and monitoring of peri-implant diseases. Further research is needed to establish standardized protocols for its clinical application and to explore its long-term predictive value in implant care.

We aimed to study the effects of fermented lingonberry juice (FLJ) as a mouthwash on the levels of active matrix metalloproteinase‐8 (aMMP‐8) in peri‐implant sulcular fluid (PISF), bleeding on probing (BOP), and visible plaque index (VPI). We hypothesized that FLJ rinsing could reduce inflammation (aMMP‐8 and BOP) and microbial load (VPI) in the oral cavity, especially around dental implants.

Background: Obesity is associated with alterations in the immune response through increased systemic inflammation. This systemic inflammatory state may increase the risk of peri-implantitis, a condition characterized by infection and tissue destruction around dental implants. Therefore, this cross-sectional clinical study aimed to investigate the association between obesity and peri-implant health. Methods: In this observational clinical study, a total of 80 patients were evaluated, including a peri-implant healthy non-obese control group (CG) (n = 20), peri-implantitis non-obese group (PG) (n = 20), peri-implant healthy obese group (OG) (n = 20), and peri-implantitis obese group (POG) (n = 20). Peri-implant clinical measurements (plaque index [PI], gingival index [GI], bleeding on probing [BOP], and probing depth [PD]) were obtained from the participants. In addition, tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and matrix metalloproteinase-8 (MMP-8) levels were measured in peri-implant crevicular fluid (PICF) samples. Results: PI, GI, BOP, and PD levels were significantly higher in the POG and PG than in the other groups (p < 0.05). PICF volume was found to be higher in the POG and PG than in the control group (p < 0.05). TNF-α levels increased significantly in all groups compared with the control group, and IL-1β levels were highest in the POG (p < 0.05). Conclusions: The findings of this cross-sectional study suggest a potential association between increased proinflammatory cytokine levels and altered peri-implant inflammatory responses in patients with obesity. Trial registration: This study was registered on ClinicalTrials.gov (Identifier: NCT07183163) on 18 September 2025 (retrospectively).

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Background: Management of completely edentulous dental patients with compromised oral condition has become a prime concern for dental professionals. Treating patients who were edentulous with an overdenture supported by osseointegrated implants and retained by small diameter attachment system is a highly valuable rehabilitation treatment for older adults. Purpose: To evaluate patients’ satisfaction by oral health–related quality of life who received maxillary implant–assisted overdentures (MIAO) constructed with OT-Equator attachment clinically as well as biochemical measuring of matrix metalloproteinase-8 (MMP-8) in the peri-implant soft tissue. Material and Methods: This study included 25 patients rehabilitated with MIAO with OT-Equator attachments that screwed to the abutments and mandibular removable partial denture restoring up to second premolars. Biochemical evaluation of collagenase-2 (MMP-8) in peri-implant soft tissue was carried out using sandwich enzyme immunoassay–enzyme-linked immunosorbent assay (ELISA) kit. Then oral health impact profile index was used as a shortened 14-item questionnaire to evaluate the impact of oral health on the quality of life. Results: Collagenase-2 (MMP-8) in the peri-implant sulcular fluid revealed a statistically significant difference in different periods (F = 56.649). Five domains showed a statistically significant improvement at 5% level (functional limitation [P = 0.001], physical pain [P = 0.037], psychological discomfort [P = 0.007], physical disability [P = 0.010], and psychological disability [P = 0.016]) except domains regarding social disability and handicap that showed no significant improvement. Five of the seven domains of the oral health related quality of life (OHIP-14) questionnaire showed a statistically significant improvement at 5% level (functional limitation [P = 0.001], physical pain [P = 0.039], psychological discomfort [P = 0.007], physical disability [P = 0.010], psychological disability P = 0.017]). Conclusion: Results showed that the patients with MIAO were satisfied with their oral health–related quality of life. Expectable and encouraging treatment outcomes among elderly patients in severe situations with bone deficiency in the maxilla can be achieved with OT-Equator attachments. Biochemical analysis of collagenase-2 (MMP-8) was a potential host marker for the condition of peri-implant tissue as it could prevent failure of dental implant.

INTRODUCTION The study evaluates the levels of matrix metalloprotease-8 (MMP-8), and Cathepsin-K (CatK) in peri-implant crevicular fluid (PICF) among patients with immediate loaded (IL) and delayed-loaded (DL) implants at different time points to know the inflammation and osteogenic status. METHODS The study population consisted of two groups (n = 25, each group) with a mean age of 28.7 ± 3.5 years, and PICF was collected. MMP-8 and CatK levels were quantified through ELISA. RESULTS We observed the concentrations of inflammatory markers (MMP-8 and CatK) at three time points in the IL and DL groups. The mean concentration of MMP-8 in the IL group was 9468 ± 1230 pg/mL, 5547 ± 1088 pg/mL, and 7248 ± 1396 pg/mL at 2 weeks, 3 months, and 12 months, respectively; while in the DL group was 10 816 ± 779.7 pg/mL, 9531 ± 1245 pg/mL, and 9132 ± 1265 pg/mL at 2 weeks, 3 and 12 months, respectively. The mean concentration of Cat-K in the IL group was observed at 422.1 ± 36.46 pg/mL, 242.9 ± 25.87 pg/mL, and 469 ± 75.38 pg/mL at 2 weeks, 3, and 12 months, whereas in the DL group was 654.6 ± 152.9 pg/mL, 314.7 ± 28.29 pg/mL, and 539.8 ± 115.1 pg/mL at 2 weeks, 3 months and 12 months, respectively. CONCLUSION In this study, the levels of CatK and MMP-8 levels decline at 12 months in both groups, and the IL group shows lower values compared to the DL group; however, no significant changes were observed after analyses were adjusted for multiple comparisons (p > 0.025). Therefore, there is not much difference observed in the inflammation process between immediate and delayed loading. (Clinical trial identifier: CTRI/2017/09/009668).

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Purpose: To compare the matrix metalloproteinase-8 (MMP-8) levels in the peri-miniscrew implant crevicular fluid (PMCF) of immediate-loaded and delayed-loaded miniscrew implants at different time intervals. Materials and Methods: Titanium orthodontic miniscrews were placed bilaterally in the attached gingiva of 15 patients between the maxillary second premolar and maxillary first molar for en masse retraction. This split-mouth study was designed to have an immediate-loaded miniscrew on one side and a delayed-loaded miniscrew on the other side that was loaded 8 days after miniscrew placement. PMCF was collected from the mesiobuccal aspects of the immediate-loaded implants at 24 hours, 8 days, and 28 days after loading, and from the delayed-loaded miniscrew implants at 24 hours and 8 days before loading and 24 hours and 28 days after loading. An enzyme-linked immunosorbent assay kit was used to assess MMP-8 levels in the PMCF samples. Unpaired t test, ANOVA F-test, and Tukey post hoc test were used to evaluate data at the P < .05 level. Results: Although there were slight alterations in the MMP-8 levels in the PMCF over time, there was no statistically significant difference in the MMP-8 levels between groups. There was a statistically significant decrease in the levels of MMP-8 between 24 hours after miniscrew placement and 28 days after loading on the delayed-loaded side (P < .05). Conclusion: The MMP-8 levels did not vary much between immediate-loaded and delayed-loaded miniscrew implants as a result of the force application. However, there was no significant difference between immediate loading and delayed loading in terms of biologic response to mechanical stress. The increase in MMP-8 levels after 24 hours post-miniscrew insertion, as well as the subsequent gradual reduction over the course of the study period in both immediate and delayed groups after loading, is probably due to the bone adapting to stimuli.

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The purpose of this prospective cohort study is to evaluate the effect of peri-implant phenotype (PPh) on the severity of peri-implant diseases and the results of non-surgical mechanical treatment (NSMT), along with calprotectin (CLP) and MMP-8(matrix metalloproteinase-8) levels. 77 implants from 39 patients were included. The implants were categorized Group-1(peri-implant mucositis), Group-2(peri-implantitis).Baseline (0. Month-PrT) clinical parameters (PD, GI, PI, BOP, CAL) and radiographic bone loss were documented, and peri-implant crevicular fluid (PICF) samples were collected. Various intruments and methodologies were employed to assess PPh components (mucosa thickness, supracrestal tissue height, keratinized mucosa) and peri-implant attached mucosa (AM). NSMT was applied to diseased implant sites. All clinical parameters were reassessed again by taking PICF samples at the 6th month-after treatment (PT). In PICF samples obtained from both groups, MMP-8 and CLP levels were evaluated using the ELISA test. PrT-PD,PrT-GI,PrT-CAL and PrT-BOP percentage values in Group-2 were significantly higher than Group-1.PrT-PD,PrTPI scores are significantly higher in thin biotype implants. All components of the PPh and AM were significantly lower in thin biotype. Intra-group time-dependent changes of MMP-8 and CLP were significant in both groups (p < 0.05). When the relationship between thin and thick biotype and biochemical parameters was evaluated, the change in PrT-PT didn’t show a significant difference (p > 0.05). PPh plays a role in influencing the severity of peri-implant diseases. However, the impact of phenotype on NSMT outcomes was similar in both groups. The PPh should be considered when planning implant surgery.

STATEMENT OF PROBLEM Custom healing abutments made of flowable composite resin have gained popularity, although the soft tissue response to composite resin has not been adequately studied. PURPOSE The purpose of this randomized controlled clinical trial was to evaluate the soft tissue response to titanium stock healing abutments and custom composite resin healing abutments by assessing clinical indices and the level of matrix metalloproteinase-8 (MMP-8) in the peri-implant crevicular fluid (PICF). MATERIAL AND METHODS A randomized controlled clinical trial was performed on 42 osseointegrated implants. The implants were divided into 2 groups: a test group comprising 21 custom composite resin healing abutments that were attached to the implants at second stage surgery and a control group comprising 21 stock titanium healing abutments. Plaque index (PL), bleeding on probing (BOP), modified gingival index (MGI), and level of MMP8 were measured at the second and fourth week after second stage surgery. Peri-implant crevicular fluid was collected by paper points at each follow-up, and the level of MMP8 was measured by an enzyme-linked immunosorbent assay kit. For statistical analysis, group comparisons used the Mann-Whitney U test, and comparisons within each group at 2 and 4 weeks used the Wilcoxon Sign Rank test. Group differences were analyzed with the Fisher exact test, and the McNemar test was used to compare percentages at 2 and 4 weeks. All tests were two-tailed (α=.05). RESULTS For the PI, no statistically significant differences were found within groups or between groups (P>.05). Bleeding on probing was positive in 14.3% of titanium abutments versus 20% of composite resin abutments at 4 weeks, with no significant difference between groups (P>.05). Similarly, the mean MGI was 0.38 ±0.5 in the control group while it was 0.4 ±0.5 in the test group, with no significant differences between groups (P>.05). The MMP8 level at 2 weeks was 11.1 ±8.65 and 13.11 ±9.29 for the control and test groups, respectively while it was 16.35 ±8.31 and 19.80 ±8.44 at 4 weeks, showing a statistically significant increase within groups (P<.05). No significant difference between groups was detected at either follow-up time point regarding MMP8 level (P>.05). CONCLUSIONS The clinical and biochemical soft tissue response to composite resin healing abutments and titanium stock healing abutments were comparable, suggesting the clinical safety of custom composite resin healing abutments.

This study aimed to assess levels of biomarkers associated with inflammation and tissue destruction in peri-implant crevicular fluid (PICF) of implants provided with customized or standard healing abutments during early implant healing. Thirty implants were placed in 22 patients with partial posterior edentulism. Subsequently, test group implants (n=15) received one-piece titanium abutments that were fabricated using computer-aided design/computer-aided manufacturing (CAD/CAM). Control group implants (n=15) were provided with standard abutments. PICF collection and standardized periapical radiographs were carried out at suture removal one week later, following crown delivery after 3 months and at 6 months. Expression of C-reactive protein (CRP), interferon-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12A, IL-17A, macrophage inflammatory protein (MIP)-1α, matrix metalloproteinase (MMP)-13, osteopontin, osteoactivin, Receptor Activator of NF-κB (RANK), and TGF-β were analyzed using a multiplex ELISA kit. Both groups showed a significant decrease in protein expression of CRP, IL-1β, IL-6, IL-8, MIP-1α, osteopontin, osteoactivin, and TGF-β, while MMP-13 levels increased during the observation period. A rise in OPG and RANK levels was detected among customized abutments. Expression of CRP was higher, whereas IL-1β, IL-1α, and MIP-1α were decreased in control compared to test group implants after 6 months. Marginal bone loss did not depend on abutment modality. Both abutment types showed distinctive temporal expression of inflammatory biomarkers during 6 months following implant placement. ISRCTN98477184, registration date 18/05/2022 Customized healing abutments exert similar effects on inflammation during early implant healing compared to standard healing abutments.

Abstract Objective The xenogeneic collagen matrix (XCM) is widely used for keratinized mucosa augmentation around natural teeth and dental implants due to its consistent efficacy and the advantage of avoiding a second surgical site. However, the shrinkage rate of XCM after surgery exceeds 50%, which is not conducive to accurate preoperative design. This study aimed to investigate factors influencing XCM shrinkage. Materials and Methods Fifteen participants with buccal keratinized tissue width (KTW) <2 mm around mandibular single implants underwent XCM-based KTW augmentation. Clinical parameters were recorded, and peri-implant crevicular fluid (PICF) was collected preoperatively and at 3-month follow-ups. Twenty cytokines in PICF were assessed using a commercial kit, with Spearman tests evaluating correlations between cytokines, clinical parameters, and shrinkage predictors. Results XCM significantly increased KTW from 1.39 ± 0.26 mm to 4.13 ± 1.19 mm at 3 months ( p  < 0.001), with a shrinkage rate of 60.12 ± 12%. The PICF showed significant decreases in C-reactive protein (CRP), interleukin-6 (IL-6), calprotectin (CLP), and keratin type II cytoskeletal 1 (K2C1) ( p  < 0.05) and a significant increase in osteoprotegerin (OPG) at 3 months ( p  < 0.05). Preoperative keratinized tissue thickness (KTT) and cathepsin K (CTSK), along with K2C1 at 3 months, correlated with XCM shrinkage. Conclusion KTW augmentation alters cytokine expression. Thin preoperative KTT and high CTSK in PICF may predict high postoperative XCM shrinkage. Clinical Relevance Gingival phenotype significantly impacts XCM shrinkage after augmentation. PICF cytokine expression could serve as a predictive biomarker. Trial Registration The Chinese Clinical Trial Registry, no. ChiCTR2500100933 (last updated on 17/04/2025).

This proof-of-concept study aimed to compare the biochemical composition of gingival crevicular fluid (GCF) and peri-implant crevicular fluid (PICF) under healthy conditions, through mid-infrared (MIR) spectroscopy. Using a split-mouth design, GCF and PICF samples were collected from 12 participants and analyzed through MIR spectroscopy. Advanced chemometric models, including partial least squares-discriminant analysis, k-nearest neighbors, and support vector machine discriminant analysis, were applied to explore potential biochemical differences between the biofluids. No cluster formation was observed with PCA, indicating a high degree of similarity between groups. The PLS-DA model didn’t effectively discriminate between GCF and PICF with prediction rates of 62.5% (10/16) for calibration, 37.5% (6/16) for cross-validation, and 50% (4/8) for validation. The k-NN model, using k = 3 neighbors showed 25% (4/16) correct classification rates during calibration and a validation set accuracy of 50%. SVM-DA analysis showed a correct prediction rate of 37.5% (6/16) for calibration and 50% for cross-validation 50% (8/16) and 50% (4/8) in the validation phase. Nonetheless, subtle spectral differences were observed in spectral regions R1 (3982–2652 cm⁻1) and R4 (1180–922 cm⁻1), suggesting a slightly increased lipidic content and the presence of ethers and glycosidic bonds linked to carbohydrates, in PICF. The lack of significant biochemical differences between GCF and PICF under healthy conditions, as determined by MIR spectroscopy, suggests that implant-related changes in PICF composition are negligible. The demonstrated biochemical similarity between GCF and PICF under healthy conditions reinforces the potential of PICF as a reliable biofluid for diagnostic applications, including monitoring oral and systemic health biomarkers, without significant influence from implant-related factors.

BACKGROUND Active matrix metalloproteinase-8 (MMP-8) enzymes are associated with inflammation and the degradation of implant-supporting tissues. A high concentration of these enzymes in the peri-implant sulcular fluid (PISF) has been associated with a pathological condition. The aim of the present study was to evaluate the ability of a chair-side MMP-8 test to individuate pathological peri-implant site. The working hypothesis is that positive response to MMP-8 test corresponds to pathological values of periodontal indexes. A secondary working hypothesis is that PISF sampling method influences the test accuracy. METHODS Fifty implants from 50 patients were evaluated during scheduled follow-up appointments in a clinical setting using the MMP-8 chair-side test and periodontal indices (probing depth, bleeding on probing, plaque index and peri-implant inflammation index). Two methods of PISF sampling were tested. RESULTS The MMP-8 test demonstrated significant diagnostic capacity and good sensitivity and accuracy regarding indices of bleeding on probing, plaque index, and peri-implant inflammation index but fair agreement. CONCLUSIONS Further studies should be performed to better define the interpretation of the MMP-8 chair-test results and their correlation to peri-implant indexes.

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Evaluation of periodontal and peri-implant tissue condition is mainly based on clinical examination and imaging diagnostics. Some data imply that Metalloproteinase-8 (MMP-8) level examination in peri-implant sulcular fluid (PISF) might be useful for evaluating the condition of peri-implant tissues and monitoring a development of peri-implant inflammation, including both mucositis and peri-implantitis. Hence, in this study, we decided to evaluate the level of MMP-8 in PISF obtained from patients without clinical symptoms of mucositis or peri-implantitis and compare it with MMP-8 level in gingival crevicular fluid (GCF) obtained from patients with healthy periodontium and those with varying severity of periodontitis. A total of 189 subjects were included in the study, and GCF/PISF samples were analysed for MMP-8 level by ELISA test. We documented that MMP-8 level in PISF obtained from patients without symptoms of mucositis or peri-implantitis was significantly higher not only than in GCF of periodontally healthy patients but also, which seems to be very interesting, than in GCF of patients with varying degrees of periodontal inflammation, consistent with earlier studies. Our observation might imply that monitoring of MMP-8 level in PISF could help to diagnose mucositis/peri-implantitis in an early stage, prior to clinical manifestations, which may allow for quick start of appropriate therapy.

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This retrospective clinical study compared two different professional maintenance therapy (MT) approaches, with and without periodic removal and decontamination of prosthetic components, on peri-implant inflammatory clinical and biochemical parameters after 5 years of implant function. MATERIALS AND METHODS A retrospective analysis based on patient records was used to assess inflammatory clinical and biochemical parameters around dental implants installed by the same clinician in a private clinic. The plaque index (PI), peri-implant probing depth (PPD), mucosa! recession (MR) bleeding on probing (BOP) and radiographic marginal bone level were determined at baseline and at each year during the follow-up period. Moreover, at last follow-up visit the peri-implant intrasulcular levels of active metalloproteinasis 8 (aMMP-8) were also assessed. All participants were under MT with a 6-month recall interval. RESULTS Ninety-two patients with 132 implants were selected. At the end of the study period, 12 patients with 12 implants were classified as "dropouts". Remained 80 patients and 120 implants were classified into two groups: Group 1, (42 patients with 62 implants) received MT with periodic removal and decontamination of prosthetic components; Group 2, (38 patients with 58 implants) received MT without periodic removal and decontamination of prosthetic components. No statistical differences were found between the groups regarding presence of plaque, probing depth, and mean mucosal recession. Group 1 presented a statistically significant higher number of sites with BOP (12.4% vs. 6.2%). Marginal bone loss was statistically higher in Group 2 than in Group 1 (0.23 ± 0.6 mm vs. and 0.78 ± 0.3 mm). Intrasulcular levels of aMMP-8 were statistically higher in Group 1 than in Group 2. CONCLUSIONS The supplemental application of periodic removal and decontamination of prosthetic components during MT had a significant additional benefit in the peri-implant tissues inflammatory status.

ABSTRACT Objectives The number of dental implants is constantly on the rise, and inflammation of their surrounding tissues is an increasing problem. In this randomized controlled trial, we investigated the effects of antibacterial dual‐light photodynamic therapy (aPDT) in peri‐implant disease in reduction of inflammation in the peri‐implant tissues. Material and Methods Forty patients with newly diagnosed peri‐implant disease were recruited. The diagnoses were established clinically and by radiological bone loss (RBL). Dual‐light aPDT was provided by indocyanine green mouth rinsing and 50:50, 405 nm, and 810 nm light applicator. The treatment group (n = 20) was randomized to use aPDT treatment at home for 4 weeks. The treatment was carried out once daily for 2 weeks, then twice daily for the next 2 weeks. The control group (n = 20) continued enhanced self‐care during the study period. Traditional measurement methods around the implant were measured at the beginning, at 2 weeks and at 4 weeks. Results During the study period, VPI decreased in both groups. In the treatment group BOP decreased from mean (SD) 4.7 ± 1.3 to 1.8 ± 1.6, p < 0.0001, but no change was observed in the control group (3.5 ± 2.3 to 3.0 ± 2.3, p = 0.39). In the aPDT treatment group aMMP‐8 decreased from mean (SD) 100 ± 41 to 72 ± 38, p = 0.027, but not in the control group (86 ± 54 to 86 ± 60, p = 0.38). Conclusions Regularly applied dual‐light aPDT reduces inflammation in the dental implant adjacent tissues. Dual‐light aPDT approach holds promise as an effective home care tool for implant patients.

Severe periodontitis is ranked as the sixth most prevalent disease affecting humankind, with an estimated 740 million people affected worldwide. The diagnosis of periodontal diseases mainly relies upon assessment of conventional clinical parameters. However, these parameters reflect past, rather than current, clinical status or future disease progression and, likely, outcome of periodontal treatment. Specific and sensitive biomarkers for periodontal diseases have been examined widely to address these issues and some biomarkers have been translated as point-of-care (PoC) tests. The aim of this review was to provide an update on PoC tests for use in the diagnosis and management of periodontal diseases. Among the PoC tests developed so far, active matrix metalloproteinase-8 has shown promising results in terms of diagnostic and prognostic values. However, further studies are required to increase the sensitivity and specificity via combining more than one biomarker and merging these test kits with periodontal risk assessment tools. Furthermore, the validity of these test kits needs to be investigated by applying the results in further independent studies and the impact on these test kits’, together with the results of risk factors for periodontal diseases, such as diabetes and smoking, also needs to be examined.

This article presents the results of a comprehensive clinical and biochemical study on the role of MMP-8 (matrix metalloproteinase-8), sRANKL (soluble RANK ligand), and osteocalcin in patients with partial secondary edentulism. The aim of the study was to evaluate changes in these markers in the oral fluid under different clinical conditions: in healthy volunteers (control group), in patients with secondary edentulism without treatment, and after receiving prosthetic care. The predominance of resorptive processes over bone formation in the alveolar ridge area can be the main cause of premature tooth loss, while inflammatory changes in the periodontal tissues accelerate disease progression. Materials and Methods of the Study. A total of 60 individuals were examined: 20 healthy volunteers (Group 1), 20 patients with partial secondary edentulism without treatment (Group 2), and 20 individuals with secondary edentulism who underwent prosthetic treatment (Group 3). Biochemical analyses were performed using immunoenzymatic methods with appropriate ELISA kits (Matrix Metalloproteinase-8, Human MMP8 ELISA Kit; Hycult Biotech for sRANKL; ThermoFisher for osteocalcin), enabling accurate measurement of marker concentrations in biological fluids. Statistical data processing was carried out using the Statistica 6.0 software package, testing for normality of distribution and applying the Kruskal-Wallis and Whitney-Mann tests. Study Results. The control group (healthy volunteers) showed MMP-8, sRANKL, and osteocalcin levels within physiological norms. In the second group of patients, there was a marked increase in MMP-8 and sRANKL combined with a significant decrease in osteocalcin compared to the control group. This combination of changes indicates intensive osteoclast activity, enhanced by inflammation. The presence of strong correlations was also confirmed: positive between MMP-8 and sRANKL and negative between these markers and osteocalcin. Such correlations suggest that when collagen destruction and bone resorption intensify (high MMP-8 and sRANKL), natural bone formation (osteoblastic activity) is suppressed. In the third group, where patients received comprehensive prosthetic treatment, MMP-8 and sRANKL levels were significantly lower than in the untreated group, while osteocalcin levels almost doubled. Although these changes did not reach the values observed in healthy volunteers, they demonstrate a clear tendency toward reducing inflammatory processes and partially normalizing bone remodeling. The obtained results highlight the potential of simultaneously measuring MMP-8, sRANKL, and osteocalcin for assessing the condition of periodontal tissues and the alveolar bone in patients with secondary edentulism. Decreasing MMP-8 and sRANKL levels against a background of increased osteocalcin confirms the effectiveness of prosthetic therapy and allows for the early detection of positive dynamics in bone remodeling processes. Thus, the approach proposed by the authors allows for the consideration of MMP-8, sRANKL, and osteocalcin as key markers reflecting both inflammatory and reparative processes in the oral cavity under conditions of secondary edentulism. The results obtained underscore the feasibility of employing prosthetic treatment methods to reduce destructive effects on bone tissue and open up new prospects for creating personalized treatment and rehabilitation algorithms for patients who have lost their teeth.

Aim: This study aimed to investigate the association between salivary levels of myeloperoxidase (MPO), neutrophil elastase (NE), soluble urokinase‐type plasminogen activator receptor (suPAR), matrix metalloproteinase (MMP)‐8 and tissue inhibitor of matrix metalloproteinases (TIMP)‐1 and gingival inflammation development during an experimental gingivitis study. Methods: A three‐week experimental gingivitis study was conducted. Clinical recordings of dental plaque biofilm (Modified Quigley Hein Plaque Index, TQHPI) and gingival inflammation (Modified Gingival Index, MGI) were made at specific time points for each of the 42 participants. Salivary levels of MPO, NE, suPAR, MMP‐8 and TIMP‐1 at the same time points were measured using distinct immunoassays. For data analysis growth curve modelling was employed to account for the time‐varying outcome (MGI score) and the time‐varying covariates (salivary marker levels, and TQHPI score). Analyses were stratified according to the MGI‐score trajectory groups previously identified as ‘fast’, respectively ‘slow’ responders. Results: Overall, higher MGI scores were statistically significantly positively associated with higher levels of MPO, MMP‐8 and TIMP‐1. Stratified analysis according to inflammation development trajectory group revealed higher levels of salivary MPO, MMP‐8 and MMP‐8/TIMP‐1 ratio among the ‘fast’ responders than among ‘slow’ responders. None of the investigated salivary protein markers was associated with a ‘slow’ inflammation development response. Conclusions: Salivary levels of MPO, MMP‐8 and TIMP‐1 were associated with the extent and severity of gingival inflammation. While the ‘fast’ gingival inflammation response was associated with increased levels of MPO, MMP‐8 and MMP‐8/TIMP‐1 ratio, the ‘slow’ response was not associated with any of the salivary protein markers investigated in this study. Neutrophil activity seems to orchestrate a ‘fast’ gingival inflammatory response among participants previously primed to gingival inflammation.

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The effect of head and neck cancer (HNC) radiotherapy (RT) on biomarkers is not known but there is a lot of potential for gaining more precise cancer treatments and less side effects. This cohort study investigated the levels and molecular forms of the matrix metalloproteinase (MMP) -8 and -9, tissue inhibitor of metalloproteinase (TIMP)-1, myeloperoxidase (MPO) and interleukin (IL)-6 in mouth-rinse samples as well as the clinical periodontal status in HNC patients (n = 21) receiving RT. Complete periodontal examinations were performed pre-RT and one month after RT. Mouth-rinse samples (pre-RT, after six weeks of RT and one month after RT) were assayed using a point-of-care-kit (PerioSafe®/ORALyzer® (Dentognostics GmbH, Jena, Germany)) for active MMP-8 and ELISA analysis for total MMP-8 and -9, MPO, TIMP-1, and IL-6 levels. Molecular forms of MMP-9 were assessed by gelatinolytic zymography and MMP-8 by western immunoblot. Significant changes were observed between the three time points in the mean levels of active and total MMP-8, active MMP-9, and IL-6. Their levels increased during the RT and decreased after the RT period. The aMMP-8 levels stayed elevated even one month after RT compared to the pre-RT. Clinical attachment loss, probing depths, and bleeding on probing were increased between pre- and post-calculations in periodontal status. Elevated inflammatory biomarker levels together with clinical recordings strongly suggest that RT eventually increases the risk to the periodontal tissue destruction by inducing the active proteolytical MMP-cascade, and especially by prolonged activity of collagenolytic aMMP-8. Eventually, the aMMP-8 point-of-care mouth-rinse test could be an easy, early detection tool for estimating the risk for periodontal damage by the destructive MMP-cascade in HNC patients with RT treatment.

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